RNA sequencing is a genomics technology that allows scientists to take a snapshot of gene expression from a single cell or population of cells at the level of single nucleotides. RNA-Seq can determine the biological response to environmental stressors or mutations and mechanisms of gene regulation. RNA is purified from cells, fragmented, and deeply sequenced. Each dataset consists of 1 billion bases and the typical experiment contains 40 datasets. Each raw data file is 1 Gb and processed datasets are 10-100 Gb. We developed a system that enables scientists to process, analyze, and visualize datasets via a web interface. The system runs on the Oklahoma State University Cloud, hosted by the OSU High Performance Computing Center. This toolbox enables scientists to deduce the roles of genes and individual gene regulation features, which is an important step in determining genome function.

Presenter(s)

Tyrell Conway